Time resolved fluorescence decay analysis: Pulse5

A sample is illuminated with a brief flash of light, and the time decay of any fluorescence is observed. The task is to estimate the number, strength and decay constants of multiple exponential decays present in the time series, taking into account the instantaneous response function (IRF) of the lamp and detector.

The figure below shows the data and IRF (top panel) and below that, the estimated lifetime distribution. In this case there are five exponential decays, of differing magnitudes. Four of the lifetimes are quite well determined, while the lifetime of the decay around 0.7ns is less well known.

: Download a demo version of Pulse5.
: Get demo instructions
: Example:Fitting 4 exponentials to mock data
: Example:Fitting 5 exponentials to real data